This project conducted initial testing to develop a method for quantifying plant and soil carbon sequestration capabilities under the controlled conditions of the Texas Transportation Institute’s Environmental and Emissions Research Facility (EERF). Plants and soil were subjected to heavy-duty truck emissions over a six-week period. Samples were analyzed for changes in carbon and nitrogen content over time. Due to the plant injury that occurred during testing, the sequestration capabilities of these plant materials and soils were inconclusive. A comparison of samples taken over the course of the study indicated that the desired results may have been accomplished had the initial exposure in the EERF been reduced to a more moderate level. Modifications to this technique for future research on specific soils and plant materials may help identify plant and soil combinations to maximize roadside carbon sequestration.